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Abstract
Aim. The aim of this study was to investigate the influence of zinc supplementation on the development of thymic involution during the growth of transplantable tumor in mice.
Materials and methods. Inbred C3HA mice after subcutaneous syngeneic hepatoma 22a inoculation received zinc sulfate with drinking water. After 3 weeks of tumor growth animals were killed, and evaluated for zinc content in serum blood samples, thymus status and parameters of tumor growth.
Results. At the 21 day of tumor 22a growth weight and cellular content of the thymus were decreased by 3 times and endogenous zinc content in samples of blood serum was decreased by 1,9 times. Oral zinc sulfate supplementation at concentration of 22 mkg/ml in drinking water during 3 weeks, started from the first day after tumor inoculation, increased thymus weight and cellularity as well as serum zinc content. At the same time, it did not influence tumor size and survival rate of mice with hepatoma 22a. As a possible mechanism of zinc action, we studied the influence of zinc supplementation on the activity of two anti-oxidative defense enzymes: Cu,Zn-superoxide dismutase and catalase in the thymus. Zinc supplementation had no influence on these parameters, both remained at a higher level in tumor-bearing mice.
Conclusion. Oral zinc supplementation in hepatoma 22a bearing mice induced retards the development of thymic involution with no influence on the tumor growth per se. The study allows to suggest that oral zinc administration may be considered as a prospective strategy for thymus reconstitution in oncology patients.
References
Carrio R Lopez DM. Insights into thymic involution in tumor-bearing mice Immunologic Research. 2013;57(1–3):106–114. 10.1007/s12026-013-8446-3
Heng TSP Chidgey AP Boyd RL. Getting back at nature: understanding thymic development and overcoming its atrophyCurrent Opinion in Pharmacology. 2010;10(4):425433. 10.1016/j.coph.2010.04.006
Lepletier A Alsharif A Chidgey AP. Inflammation and thymus ageing Frontiers of Hormone Research. 2017;48:1936. 10.1159/000452903
Dardenne M Boukaiba N Gagneraout M-C et al. Restoration of the thymus in ageing mice by in vivo zinc supplementation Clinical Immunology and Immunopathology. 1993;66(2):127135. 10.1006/clin.1993.1016
Mocchegiani E Santarelli L Muzzioli M Fabris N. Reversibility of the thymus involution and of age-related peripheral immune dysfunction by zinc supplementation in old mice International Journal of Immunopharmacology. 1995;17(9):703718. 10.1016/0192-0561(95)00059-b
Wong CP Song Y Elias VD et al. Zinc supplementation increases zinc status and thymopoiesis in aged mice The Journal of nutrition. 2009;139(7):13931397. 10.3945/jn.109.106021
Mocchegiani E Santarelli L Costarelli L et al. Plasticity of neuroendocrine-thymus interactions during ontogeny and ageing: role of zinc and arginine Ageing research reviews. 2006;5(3):281309. 10.1016/j.arr.2006.06.001
John E Laskow TC Buchser WJ et al. Zinc in innate and adaptive tumor immunity Journal of Translational Medicine. 2010;8:118. 10.1186/1479-5876-8-118
Romeu A Arola L Alemany M. Essential metals in tissues and tumor of inbred C57BL/6 mice during the infective cycle of Lewis lung carcinoma Cancer Biochemistry Biophysics. 1986;9(1):5366
Eide DJ. The oxidative stress of zinc deficiency Metallomics: integrated biometal science. 2011;3(11):11241129. 10.1039/c1mt00064k
Hadwan MH Ali SK. New spectrophotometric assay for assessments of catalase activity in biological samples Analytical Biochemistry. 2018;542:2933. 10.1016/j.ab.2017.11.013
Spitz DR Oberley LW. An assay for superoxide dismutase activity in mammalian tissue homogenates Analytical Biochemistry. 1989;179(1):818. 10.1016/0003-2697(89)90192-9
Samygina VR Sokolov AV Bourenkov G et al. Rat ceruloplasmin: new labile copper binding site and zinc/copper mosaic Metallomics: integrated biometal science. 2017;9(12):18281838. 10.1039/c7mt00157f
Kaiserlian D Savino W Hassid J Dardenne M. Studies of the thymus in mice bearing the Lewis lung carcinoma. III. Possible mechanisms of tumor-induced thymic atrophy Clinical Immunology and Immunopathology. 1984;32(3):316325. 10.1016/0090-1229(84)90275-7
Mariani E Mangialasche F Feliziani FT et al. Effects of zinc supplementation on antioxidant enzyme activities in healthy old subjects Experimental Gerontology. 2008;43(5):445451. 10.1016/j.exger.2007.10.012
Tate DJ Miceli MV Newsome DA. Zinc induces catalase expression in cultured fetal human retinal pigment epithelial cells Current Eye Research. 1997;16(10):10171023. 10.1076/ceyr.16.10.1017.9011
Hoang BX Han B Shaw DG Nimni M. Zinc as a possible preventive and therapeutic agent in pancreatic, prostate, and breast cancer European Journal of Cancer Prevention. 2016;25(5):457461. 10.1097/CEJ.0000000000000194
Ribeiro SM Braga CB Peria FM. et al. Effects of zinc supplementation on fatigue and quality of life in patients with colorectal cancer Einstein. 2017;15(1):2428. 10.1590/S1679-45082017AO3830
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