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Abstract
The current paper is dedicated to the research of the differential expression of blood leukocyte fraction (LF) genes in patients with colorectal cancer (CRC) and polyposis of the lower part of the gastrointestinal tract (GIT).
Aim. Determination of gene expression profiles in peripheral blood LF cells in patients with CRC and polyps of the lower GIT, and creation of logistic regression models, which would make it possible to differentiate patients with CRC, polyposis.
Material and methods. Patients diagnosed with CRC (n=33), polyposis (n=22), as well as control group (n=30) were included in the study.
Gene expression was determined by means of the NGS method. Libraries were prepared with the use of the RNAHyperPrep&HyperCap kit.
NGS was performed on the MiSeq sequencer.
Results. Groups of differentially expressed genes were identified, and logistic regression models were built to differentiate patients diagnosed with CRC (accuracy 93.7%) and polyposis (accuracy 84.6%), in comparison with the control group, as well as patients diagnosed with CRC from patients with polyposis with the accuracy up to 78.3%.
Conclusion. It is necessary to study the expression of genes with the highest diagnostic potential in patients from an independent sampling, using digital RT-PCR, to validate and advance the models we have created.
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