The evaluation of proliferative activity of tumor cells based on the technology of longterm phase-contrast microscopy technology CELL-IQ
Vol. 60 No. 5 (2014), Articles
Vol. 60 No. 5 (2014)

The evaluation of proliferative activity of tumor cells based on the technology of longterm phase-contrast microscopy technology CELL-IQ

Articles
https://doi.org/10.37469/0507-3758-2014-60-5-590-595
Published 15.12.2014
N.N. Petrov Research Institute of Oncology
N.N. Petrov Research Institute of Oncology
N.N. Petrov Research Institute of Oncology
N.N. Petrov Research Institute of Oncology
PDF (Русский)

Keywords

phase-contrast microscopy
live-cell imaging
proliferative activity
tumor cells
modeling

How to Cite

, , , & . (2014). The evaluation of proliferative activity of tumor cells based on the technology of longterm phase-contrast microscopy technology CELL-IQ. Voprosy Onkologii, 60(5), 590–595. https://doi.org/10.37469/0507-3758-2014-60-5-590-595
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Abstract

This work presents results of long-term phase-contrast microscopy research of proliferative potential of tumor cell lines utilizing live-cell imaging technology Cell-IQ (Chip-Man Technologies Ltd, Finland). It was found that the machine vision technology allowed to obtain sufficient body of evidence about high-quality and quantitative changes of proliferative activity of the tumor cells cultivated in static conditions. The present study demonstrates that modeling of time interval of maximum proliferative activity of tumors cells increases information efficacy and reliability of the analysis of dividing cell patterns using Cell-IQ technology. The models of exponential growth of various tumor cell lines, describing their quantitative and dynamic changes of expansion potential have been received. Modeling of maximum tumor cells proliferative activity can be applied for development of test-system of individual cell sensitivity to anticancer drugs in vitro.
https://doi.org/10.37469/0507-3758-2014-60-5-590-595
PDF (Русский)

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