How to Cite
Abstract
Aspergillus fumigatus is one of the most prevalent fungal pathogens has a worldwide distribution in patients with hematological malignancy. The patients infected with A. fumigatus have a high risk of the development of various diseases including often fatal invasive aspergillosis (IA). The insufficiency of traditional cultural and enzyme-linked immunosorbent assay (ELISA) methods in diagnostics of IA makes some investigators to apply more sensitive technologies, such as PCR. Recently new approaches to standardization of quantitative aspergillosis evaluation by using PCR were elucidated. It is allowed to carry out comparative definition of aspergillosis in hematological patients by using the both tests, PCR and ELISA Platelia Aspergillus. Analysis showed expediency of the combined test for detection of galactomannan antigen and DNA target copies of A. fumigatus. Moreover, Q-PCR assay is able to improve the diagnosis of IA in hematological malignancy patients including those who underwent high-dose chemotherapy and/or hematopoietic stem cell transplant (HSCT).References
Аравийский Р.А., Климко Н.Н., Васильева Н.В. Диагностика микозов / СПб: Издательский дом СПбМАПО. - 2004. - 186 с.
Богданов К.В., Игнатьева С.М. К вопросу об основных методах экстракции и молекулярного анализа ДНК микромицетов // Клиническая лабораторная диагностика. - 2009. - № 10. - С. 42-44.
Latge J.P. Aspergillus fumigatus and aspergillosis // Clin. Microbiol. Rev. - 1999. - Vol. 12. - P. 310-350.
Perfect J.R., Cox G.M., Lee J.Y. et al. The impact of culture isolation of Aspergillus species: a hospital-based survey of aspergillosis // Clin. Infect. Dis. - 2001. -Vol. 33. - P. 1824-33.
Wald A., Leisenring W., van Burik J.A., et al. Epidemiology of Aspergillus infections in a large cohort of patients undergoing bone marrow transplantation // J. Infect. Dis. -1997. - Vol. 175. - P. 1459-66.
Becker M.J. de Marie S., Willemse D., et al. Quantitative galactomannan detection is superior to PCR in diagnosing and monitoring invasive pulmonary aspergillosis in an experimental rat model // J. Clin. Microbiol. - 2000. - Vol. 38. - P. 1434-438.
Sanford J.P. The enigma of candiduria: evolution of bladder irrigation with amphotericin B for management--from Anecdote to Dogma and a lesson from Machiavelli // Clin. Infect. Dis. - 1993. - Vol. 16. - P. 145-147.
Giacchino M., Chiapello N., Bezzio S., et al. Aspergillus galactomannan enzyme-linked immunosorbent assay crossreactivity caused by invasive Geotrichum capitatum // J. Clin. Microbiol. - 2006. - Vol. 44. - P. 3432-3434.
Kirkpatrick C.H., Windhorst D.B. Mucocutaneous candidiasis and thymoma // Am. J. Med. - 1979. - Vol. 66. - P. 939-945.
Kami M., Fukui T., Ogawa S., et al. Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis // Clin. Infect. Dis. - 2001. - Vol. 33. - P. 1504-1512.
Hummel M., Spiess B., Cornely O.A., et al. Aspergillus PCR testing: results from a prospective PCR study within the AmBiLoad trail // Eur. J. Haematol. - 2010. - Vol. 85. - P. 164-169.
Hebart H., Löffler J., Meisner C., Serey F., et al. Early detection of aspergillus infection after allogeneic stem cell transplantation by polymerase chain reaction screening // J. Infect. Dis. - 2000. - Vol. 181. - P. 1713-1719.
Harrison E., Stalhberger T., Whelan R., et al. Aspergillus DNA contamination in blood collection tubes // Diagn. Microbiol. Infect. Dis. - 2010. - Vol. 67. - P. 392-394.
Suarez F., Lortholary O., Buland S., et al. Detection of circulating Aspergillus fumigatus DNA by real-time PCR assay of large serum volumes improves early diagnosis of invasive aspergillosis in high-risk adult patients under hematologic surveillance // J. Clin. Microbiol. - 2008. - Vol. 46. - P. 3772-3777.
Khot P.D., Ko D.L., Hackman R.C., et al. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid // BMC Infect. Dis. - 2008. - Vol. 8.-doi: 10.1186/1471-2334-8-73.
White P.L., Bretagne S., Klingspor L., et al. Aspergillus PCR: one step closer to standardization // J. Clin. Microbiol. - 2010. - Vol. 48. - P. 1231-1240.
Iwen P.C., Hinrichs S.H., Rupp M.E. Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. // Med. Mycol.-2002. - Vol. 40. - P. 87-109.
Challier S., Boyer S., Abachin E., Berche P. Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis // J. Clin Microbiol. - 2004. - Vol. 42. - P. 844-846.
Buchheidt D., Hummel M., Schleiermacher D., et al. Prospective clinical evaluation of a Light-Cycler-mediated polymerase chain reaction assay, a nested-PCR assay and a galactomannan enzyme-linked immunosorbent assay for detection of invasive aspergillosis in neutropenic cancer patients and haematological stem cell transplant recipients // Br. J. Haematol. - 2004. - Vol. 125. - P. 196-202.
De Pauw B., Walsh T.J., Donnelly J.P., et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Groups and the National Institute of Allergy and Infection Diseases Mycoses Study Group (EORTC/MSG) Consensus Group // Clin. Infect. Dis. - 2008. - Vol. 46. - P. 813-821.
Löffler J., Hebart H., Schumacher U., et al. Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood // J. Clin. Microbiol. - 1997. - Vol. 35. - P. 3311-3312.
Gobert P., Buisson J.C., Vagner O., et al. Detection of Cryptosporidium parvum DNA in formed human feces by a sensitive PCR-based assay including uracil-N-glyco-sylase inactivation // J. Clin. Microbiol. - 1997. - Vol. 35. - P. 254-256.
Maertens J.A., Klont R., Masson C., et al. Optimization of the cutoff value for the Aspergillus double-sandwich enzyme immunoassay // Clin Infect. Dis. - 2007. - Vol. 44. - P. 1329-1336.
White P.L., Linton C.J., Perry M.D., et al. The evolution and evalution of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting // Clin. Infect. Dis. - 2006. - Vol. 42. - P. 479-486.
Singh N., Paterson D.L. Aspergillus infections in transplant recipients // Clin. Microbiol. Rev. - 2005. - Vol. 18. - P. 44-69.
Einsele H., Hebart H., Kauffmann-Schneider C., et al. Risk factors for treatment failures in patients receiving PCR-based preemptive therapy for CMV infection // BMT. - 2000. - Vol. 25. - P. 757-763.
All the Copyright statements for authors are present in the standart Publishing Agreement (Public Offer) to Publish an Article in an Academic Periodical 'Problems in oncology' ...