Application of GLAD-PCR analysis for the methylation sites detection in the regulatory areas of tumor-suppressor genes ELMo1 and EsR1 in colorectal cancer
Vol. 62 No. 1 (2016), Articles
Vol. 62 No. 1 (2016)

Application of GLAD-PCR analysis for the methylation sites detection in the regulatory areas of tumor-suppressor genes ELMo1 and EsR1 in colorectal cancer

Articles
https://doi.org/10.37469/0507-3758-2016-62-1-117-121
Published 15.12.2016
State Research Center of Virology and Biotechnology “Vector"
State Research Center of Virology and Biotechnology “Vector"
State Research Center of Virology and Biotechnology “Vector"
State Research Center of Virology and Biotechnology “Vector"
State Research Center of Virology and Biotechnology “Vector"
State Research Center of Virology and Biotechnology “Vector"
Seversk Biophysical Research Centre
Seversk Biophysical Research Centre
Seversk Biophysical Research Centre
Seversk Biophysical Research Centre
State Research Center of Virology and Biotechnology “Vector"
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PDF (Русский)

Keywords

DNA methylation
tumor-suppressor genes
colorectal cancer
PCR

How to Cite

, , , , , , , , , , & . (2016). Application of GLAD-PCR analysis for the methylation sites detection in the regulatory areas of tumor-suppressor genes ELMo1 and EsR1 in colorectal cancer. Voprosy Onkologii, 62(1), 117–121. https://doi.org/10.37469/0507-3758-2016-62-1-117-121
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Abstract

Aberrant methylation of regulation regions of tumorsuppressor genes is showed for many cancer diseases. In course of this modification an enzyme DNMT3 methylates RCGY sites in CpG-islands of regulation regions producing R(5mC)GY sites. Earlier we developed GLAD-PCR assay to determine R(5mC)GY site in a definite position of human genome. In this work we have applied GLAD-PCR assay to determine R(5mC)GY sites in regulation regions of ESR1 and ELMO1 tumor-suppressor genes. We have studied a fragment of first exon of ELMO1 gene and a part of ESR1 promoter region in DNA preparations from malignant cell line SW837 and colorectal tumor samples. We have checked four sites in each region and found two highly methylated sites: GCGC in first exon of ELMO1 gene and GCGT in promoter region of ESR1 gene. Site GCGT is weakly methylated in healthy tissues and more methylated in the most of colorectal samples. Site GCGC is not methylated in healthy tissues and significantly methylated in 60% of colorectal samples. A possibility to use GLAD-PCR assay for cancer diagnostics is discussed.
https://doi.org/10.37469/0507-3758-2016-62-1-117-121
##article.numberofdownloads## 101
##article.numberofviews## 117
PDF (Русский)

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