Kruglov, S. S., Panchenko, A. V., Nekhaeva, T. L., Tyndyk, M. L., & Fedoros, E. I. (2023). Efficiency of the Combined Photodynamic Therapy with Cisplatin in the Treatment of B16 Melanoma in C57BL/6 Mice. Voprosy Onkologii, 69(5), 850–854. https://doi.org/10.37469/0507-3758-2023-69-5-850-854

Abstract

Introduction. Photodynamic therapy is one of the modern methods for treating tumor and pre-tumor diseases. This method has several limitations, which may be overcome by combining it with chemotherapeutic antitumor agents.

Aim. To investigate the potential of combining photodynamic therapy with cisplatin in a B16 melanoma model in C57BL/6 mice.

Materials and methods. We evaluated the possibility of combining photodynamic antitumor treatment using the chlorine photosensitizer Radachlorin® and cisplatin in a B16 melanoma model in C57BL/6 mice. Photoactivation was performed using 662 nm laser irradiation at a dose of 300 J/cm².

Results. Tumor growth inhibition compared to the control group was maximal at 7 days and amounted to 42 % in the photodynamic therapy group (p < 0.01), 60 % in the cisplatin group administered 30 minutes before photodynamic therapy (p < 0.001), 57 % in the cisplatin group administered 24 hours after photodynamic therapy (p < 0.001), and 31 % (p < 0.05) in the cisplatin-only group. The mean survival time of animals until the tumor reached a volume of 4 cm³ was 9.8 ± 0.6 days in the control group, 9.3 ± 1.7 days in the photodynamic therapy group (p = 0.9829), 8.9 ± 0.8 days in the cisplatin group (p = 0.3247), 10.3 ± 2.8 days when cisplatin was administered 30 minutes before photodynamic therapy (p = 0.1309), and 12.7 ± 2.1 days when cisplatin was administered 24 hours after photodynamic therapy (p = 0.0214).

Conclusion. The combined use of Radachlorin® photodynamic therapy with cisplatin in the B16 melanoma model resulted in an additive anti-tumor effect and an increase in survival.

https://doi.org/10.37469/0507-3758-2023-69-5-850-854

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