How to Cite
Abstract
The aim of the study was to investigate the features of the relation to the number and the phenotype of the cytotoxic T-lymphocytes and NKT-cell from regulatory T-cells content in the blood by patients with renal cell carcinoma. The study included patients with renal cell carcinoma (T3N0M0, clear cell type) at the age of 40-55 years before surgery. Lymphocyte immunophenotyping was performed by flow cytometry. It is found that in the peripheral blood of the patients with renal cell carcinoma accompanied by increased number of T-regulatory cells observed decrease content of the cytotoxic T-lymphocytes and increased levels of the NKT-cells. It is assumed that no change in the number of activated T-regulatory cells and cytotoxic T-lymphocyte determined migration from the blood. Increasing the amount of the NKT-cells in renal cancer is determined by the increase of activated and effector cells but at lower levels of the regulatory subpopulation. The content of the T-regulatory cells in healthy people weakly correlated with the effector subpopulations of T-lymphocytes. In patients with renal cancer the number of the activated T-regulatory cells is closely correlated with the various NKT-lymphocytes fractions. Moreover, if the mature and regulatory NKT-cells subset detected negative relations, so with the NKT-cells expressing CD28 and CD57 markers found positive correlations that characterizes the codirectional dynamics the activated of the regulatory and effector T-lymphocyte subpopulations levels in the background of tumor growth. A canonical analysis demonstrated that the highest significance kidney cancer patients have activated regulatory T-cells, cytotoxic T cells and NKT-cells. A canonical analysis demonstrated that the highest significance by renal cancer patients have activated regulatory T-cells, cytotoxic T-cells and NKT-cells.
References
Жулай Г.А., Олейник Е.К. Регуляторные T-клетки и канцерогенез // Иммунология. - 2013. - № 1. - С. 61-64.
Кадагидзе З.Г., Черткова А.И., Славина Е.Г. NKT-клетки и противоопухолевый иммунитет // Российский биотерапевтический журнал. - 2011. - Т 10. - № 3. - С. 9-15.
Кадагидзе З.Г., Черткова А.И., Славина Е.Г. Регуляторные Т-клетки и их роль в противоопухолевом иммунном ответе // Вопросы онкологии. - 2009. - Т. 55. - № 3. - С. 269-277.
Кудрявцев И.В., Субботовская А.И. Опыт измерения параметров иммунного статуса с использованием шестицветного цитофлуоримерического анализа // Медицинская иммунология. - 2015. - Т. 17. - № 1. - С. 19-26.
Савченко А.А., Борисов А.Г., Кудрявцев И.В., Мошев А.В. Роль Т- и В-клеточного иммунитета в патогенезе онкологических заболеваний // Вопросы онкологии. - 2015. - Т. 61. - № 6. - С. 867-875.
Савченко А.А., Модестов А.А., Мошев А.В. и др. Цито-метрический анализ NK- и NKT-клеток у больных почечноклеточным раком // Российский иммунологический журнал. - 2014. - Т. 8 (17). - № 4. - С. 1012-1018.
Хайдуков С.В., Байдун Л.А., Зурочка А.В., Тотолян Арег А. Стандартизованная технология «Исследование суб-популяционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов» (Проект) // Медицинская иммунология. - 2012. - Т. 14. - № 3. - С. 255-268.
Banerjee A., Vasanthakumar A., Grigoriadis G. Modulating T regulatory cells in cancer: how close are we? // Immunol. Cell Biol. - 2013. - Vol. 91. - P. 340-349.
Emoto M., Yoshida T., Fukuda T. et al. Alpha-galactosylce-ramide promotes killing of Listeria monocytogenes within the macrophage phagosome through invariant NKT-cell activation // Infect. Immun. - 2010. - Vol. 78. - P. 2667 -2676.
Esensten J.H., Helou YA., Chopra G. et al. CD28 Costimulation: From Mechanism to Therapy // Immunity. - 2016. - Vol. 44. - № 5. - P. 973-988.
Kared H., Martelli S., Ng T.P. et al. CD57 in human natural killer cells and T-lymphocytes // Cancer Immunol. Immu-nother. - 2016. - Vol. 65. - № 4. - P. 441-452.
Lin D., Lei L., Zhang Y et al. Secreted IL-1 promotes T-cell activation and expansion of CD11b( ) Gr1( ) cells in carbon tetrachloride-induced liver injury in mice // Eur. J. Immunol. - 2015. - Vol. 45. - № 7. - P. 2084-2098.
Liu S., Sun X., Luo J. et al. Effects of radiation on T regulatory cells in normal states and cancer: mechanisms and clinical implications // Am. J. Cancer Res. - 2015. - Vol. 15. - P. 3276-3285.
Maecker H., McCoy P., Nussenblatt R. Standardizing immunophenotyping for the human immunology project // Nat. Rev. Immunol. - 2012. - Vol. 12. - P. 191-200.
Onyema O.O., Njemini R., Forti L.N. et al. Aging-associated subpopulations of human CD8 T-lymphocytes identified by their CD28 and CD57 phenotypes // Arch. Gerontol. Geriatr. - 2015. - Vol. 61. - № 3. - P. 494-502.
Porciello N., Tuosto L. CD28 costimulatory signals in T lymphocyte activation: Emerging functions beyond a qualitative and quantitative support to TCR signaling // Cytokine Growth Factor Rev. - 2016. - Vol. 28. - P. 11-19.
Schildberg F.A., Klein S.R., Freeman G.J., Sharpe A.H. Coinhibitory Pathways in the B7-CD28 Ligand-Receptor Family // Immunity. - 2016. - Vol. 44. - № 5. - P. 955972.
Schlub T.E., Badovinac V.P., Sabel J.T. et al. Predicting CD62L expression during the CD8 T-cell response in vivo // Immunol. Cell Biol. - 2010. - Vol. 88. - № 2. - P. 157-164.
Tselios K., Sarantopoulos A., Gkougkourelas I., Boura P. T regulatory cells: a promising new target in atherosclerosis // Crit. Rev. Immunol. - 2014. - Vol. 34. - P. 389-397.
Wang H., Feng D., Park O. Et al. Invariant NKT cell activation induces neutrophil accumulation and hepatitis: opposite regulation by IL-4 and IFN-y // Hepatology. - 2013. - Vol. 58. - P. 1474-1485.
Yang S., Liu F., Wang Q.J. et al. The Shedding of CD62L (L-Selectin) Regulates the Acquisition of Lytic Activity in Human Tumor Reactive T Lymphocytes // PloS oNE. - 2011. - Vol. 6. - E22560.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
© АННМО «Вопросы онкологии», Copyright (c) 2017