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NO-synthase activity and NO level in the mitochondrial fraction of rats Guerin’s carcinoma under conditions of different ю-3 and ю-6 polyunsaturated fatty acids (PUFAs) supplementation has been investigated. Guerin’s carcinoma was used as a cancer model. 0.5 ml of 30% carcinoma cell suspension in normal saline was implanted subcutaneously into thigh of a hind leg. The intensity of tumor growth was evaluated based on measurements of subcutaneous tumor size and coefficient of tumor mass. It was showed that ω-3 PUFAs supplementation before and after transplantation of Guerin’s carcinoma resulted in the decreases of tumor growth and coefficient of tumor mass in an organism. Additional ю-6 PUFA supplementation can stimulate tumor progression and increase tumor mass in body in the logarithmic phases of carcinogenesis as compared to the tumor-bearing rats. The effect depends on duration of ю-3 and ю-6 PUFAs administration and on the level of preliminary provision of an organism with these PUFAs. We found increased NO-synthase activity and NO content in mitochondrial fraction of Guerin’s carcinoma in animals of the group that was administered ю-3 PUFAs both before and post-implantation of the carcinoma during logarithmic phase of carcinogenesis. At the same time, the ю-6 PUFA administration does not contribute to significant changes in the NO-synthase system components in the mitochondrial fraction of Guerin’s carcinoma in comparison with the tumor-bearing rats.References
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