Human papillomavirus in malignant neoplasms of various localizations
Vol. 69 No. 1 (2023), ORIGINAL ARTICLES. C. CLINICAL RESEARCH
Vol. 69 No. 1 (2023)

Human papillomavirus in malignant neoplasms of various localizations

ORIGINAL ARTICLES. C. CLINICAL RESEARCH
https://doi.org/10.37469/0507-3758-2023-69-1-89-94
Published 07.03.2023
Dmitriy V.
St. Petersburg Pasteur Institute
https://orcid.org/0000-0002-1268-6172
А.
St. Petersburg Pasteur Institute
https://orcid.org/0000-0001-9140-8957
Ludmila V.
St. Petersburg Pasteur Institute; North-Western State Medical University named after I.I. Mechnikov
https://orcid.org/0000-0001-9921-3505
А.
Saint-Petersburg state budgetary institution of health care “Saint-Petersburg City Oncology Clinic”
Sergey V.
Saint-Petersburg state budgetary institution of health care “Saint-Petersburg City Oncology Clinic”
https://orcid.org/0000-0002-0522-1772
V.
St. Petersburg Pasteur Institute
https://orcid.org/0000-0002-0830-5808
Eldar E.
North-Western State Medical University named after I.I. Mechnikov, Saint-Petersburg state budgetary institution of health care “Saint-Petersburg City Oncology Clinic”
https://orcid.org/0000-0002-1700-1128
pdf (Русский)

Keywords

malignant neoplasms
human papillomavirus
viral load
physical status
genotype

How to Cite

Kholopov, D., Vyazovaya, A., Lyalina, L., Alexeyeva, D., Molchanov, S., Narvskay, O., & Topuzov, E. (2023). Human papillomavirus in malignant neoplasms of various localizations. Voprosy Onkologii, 69(1), 89–94. https://doi.org/10.37469/0507-3758-2023-69-1-89-94
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Abstract

Introduction.  High-risk human papillomaviruses (HPVs) which include dozens of genotypes (16, 18, 45, etc.) are detected in some malignant tumors.

Aim.  To determine the viral load, genotypes and DNA status for HPVs in HPV-positive tumour tissue samples taken from patients with malignant neoplasms of different localisation.

Materials and methods. A total of 61 HPV-positive tumor tissue samples from patients with oropharyngeal, anal cancer and cervical malignancies were examined. Identification, genotyping, viral load and detection of physical status of HPV-DNA were carried out by real-time PCR using commercially available domestic test kits.

Results. All samples showed the presence of HPV genotype 16: in 86.9% (53/61) as a monogenotype and in the rest as combinations with 18, 31, 35, 39, 45. A high HPV viral load was detected in 60.7% of cases, mainly in anal and cervical cancers. A partially integrated (‘mixed’) viral DNA was detected in 97.4% (37/38) of samples with high viral load, while a fully integrated viral DNA was detected in 83.3% (5/6) of samples with low viral load (p=0.00003).

Conclusion. DNA of HPV genotype 16 partially or fully integrated into the human genome was detected in all HPV-positive tumour tissue samples of malignant neoplasms of different localizations. The fully integrated form was detected in samples with low viral load in both early and late stages of cancer.

https://doi.org/10.37469/0507-3758-2023-69-1-89-94
pdf (Русский)

References

Wild CP, Weiderpass E, Stewart BW, et al. World Cancer Report: Cancer Research for Cancer Prevention. Lyon, France: International Agency for Research on Cancer. 2020. Available from: http://publications.iarc.fr/586.

Vanden Broeck D, et al. Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective [Internet]. London: IntechOpen; 2012:362. Available from: https://www.intechopen.com/books/2054. doi:10.5772/2464.

Вязовая А.А., Куевда Д.А., Трофимова О.Б., и др. Выявление вирусов папилломы человека высокого канцерогенного риска и оценка физического статуса вирусной ДНК методом полимеразной реакции при поражении цервикального эпителия. Клиническая лабораторная диагностика. 2013;(8):24-26 [Vyazovaуа AА, Kuevda DA, Trofimova OB, et al. Detection of high carcinogenic risk human papillomaviruses and assessment of physical status of viral dna by polymerase chain reaction in cervical epithelium lesions. Russian Clinical Laboratory Diagnostics. 2013;(8):24-26 (In Russ.)].

Ибрагимова М.К., Цыганов М.М., Карабут И.В., и др. Интегративная и эписомальная формы генотипа 16 вируса папилломы человека при цервикальных интраэпителиальных неоплазиях и раке шейки матки // Вопросы вирусологии. 2016;61(6):270-274 [Ibragimova MK, Tsyganov MM, Karabut IV, et al. Integrative and episomal forms of genotype 16 of human papillomavirus in patients with cervical intraepithelial neoplasia and cervical cancer. Problems of Virology. 2016;61(6):270-4 (In Russ.)]. doi:10.18821/0507-4088-2016-61-6-270-274.

Zuo J, Huang Y, An J, et al. Nomograms based on HPV load for predicting survival in cervical squamous cell carcinoma: An observational study with a long-term follow-up. Chin J Cancer Res. 2019;31(2):389-399. doi:10.21147/j.issn.1000-9604.2019.02.13.

Киселева В.И., Мкртчян Л.С., Иванов С.А., и др. Наличие интеграции ДНК вируса папилломы человека 16-го типа и прогноз неблагоприятного исхода рака шейки матки III стадии. // Бюллетень экспериментальной биологии и медицины. 2019;168(7):100-105 [Kiseleva VI, Mkrtchyan LS, Ivanov SA, et al. The presence of human papillomavirus dna integration is associated with poor clinical results in patients with third-stage cervical cancer. Bull Exp Biol Med. 2019;168(1):87-91 (In Russ.)]. doi:10.1007/s10517-019-04654-2.

Olthof NC, Speel EJ, Kolligs J, et al. Comprehensive analysis of HPV16 integration in OSCC reveals no significant impact of physical status on viral oncogene and virally disrupted human gene expression. PLoS ONE. 2014;9:e88718. doi:10.1371/journal.pone.0088718.

Hashida Y, Higuchi T, Matsumoto S, et al. Prognostic significance of human papillomavirus 16 viral load level in patients with oropharyngeal cancer. Cancer Sci. 2021;112(10):4404-4417. doi:10.1111/cas.15105.

Morel A, Neuzillet C, Wack M, et al. Mechanistic signatures of human papillomavirus insertions in anal squamous cell carcinomas. Cancers. 2019;11(12):1846. doi:10.3390/cancers11121846.

Deng T, Feng Y, Zheng J, et al. Low initial human papillomavirus viral load may indicate worse prognosis in patients with cervical carcinoma treated with surgery. J Gynecol Oncol. 2015;26(2):111-117. doi:10.3802/jgo.2015.26.2.111.

Valmary-Degano S, Jacquin E, Prétet J-L, et al. Signature patterns of human papillomavirus type 16 in invasive anal carcinoma. Hum Pathol. 2013;44(6):992-1002. doi:10.1016/j.humpath.2012.08.019.

Shukla S, Jadli M, Thakur K, et al. Level of phospho-STAT3 (Tyr705) correlates with copy number and physical state of human papillomavirus 16 genome in cervical precancer and cancer lesions. PLoS ONE. 2019;14(9):e0222089. doi:10.1371/journal.pone.0222089.

Del Río-Ospina L, Soto-De León SC, Camargo M, et al. The DNA load of six high-risk human papillomavirus types and its association with cervical lesions. BMC Cancer. 2015;15(100). doi:10.1186/s12885-015-1126-z.

Olthof NC, Straetmans JMJAA, Snoeck R, et al. Next generation treatment strategies for HPV-related head and neck squamous cell carcinomas: where do we go? Rev Med Virol. 2012;22:88-105. doi:10.1002/rmv.714.

Olthof NC, Huebbers CU, Kolligs J, et al. Viral load, gene expression andmapping of viral integration sites in HPV16-associated HNSCC cell lines. Int J Cancer. 2015;136:е207-е218. doi:10.1002/ijc.29112.

Parfenov M, Pedamallu CS, Gehlenborg N, et al. Characterization of HPV and host genome interactions in primary head and neck cancers. Proc. Natl. Acad. Sci. USA. 2014;111:15544-15549. doi:10.1073/pnas.1416074111.

Balaji H, Demers I, Wuerdemann N, et al. Causes and consequences of HPV integration in head and neck squamous cell carcinomas: State of the Art. Cancers (Basel). 2021;13(16):4089. doi:10.3390/cancers13164089.

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