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Abstract
Introduction. Experimental studies in mammals indicate a possible risk of inheritance of disorders caused by parental irradiation in the offspring, not only of the first but also of the second generation. Therefore, the search for means to mitigate this effect is an important task.
Aim. To evaluate the transgenerational effect of the polyphenolic composition BP-C2 on tumor formation in F2 generation of Danio rerio (zebrafish) originated from irradiated males.
Materials and Methods. The F2 generation was obtained by crossing an intact female with F1 generation males originating from males exposed to 5 Gy X-rays and treated or not in a solution of the BP-C2 composition, as well as from control intact males.
F2 generation fish at the age of 10 months were exposed to N-diethylnitrosamine (100 ppm) for 20 days. Euthanasia was performed 6 months after the end of exposure to the carcinogen and the frequency of different types of liver tumors was assessed after histological processing.
Results. In the F2 generation progeny of irradiated fish, the incidence of hepatocellular carcinoma increased by 2.75-fold (p = 0.0213), total tumors by 1.86-fold (p = 0.0329) and malignant tumors by 2.17-fold (p = 0.0128) compared with the progeny of intact fish. The use of BP-C2 in F0 males after irradiation led to a decrease in the number of malignant tumors to the control level (p = 0.7300) and to a change in the structure of the tumor pathology: a decrease in the incidence of hepatocellular carcinomas was observed, as well as a tendency to an increase in the incidence of liver adenomas and cholangiocarcinomas compared to the other groups.
Conclusion. It was found that transgenerational effects of radiation exposure in Danio rerio fish persist in the second generation offspring of irradiated males. At the same time, the use of the polyphenolic composition BP-C2 immediately after irradiation reduced the severity of DENA-induced liver carcinogenesis in the offspring.
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