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Abstract
Since chronic neurogenic pain has been reported to affect biological characteristics of B16/F10 melanoma, the purpose of the study was to analyze concentrations of components of the NO-system in mice during the growth of transplantable B16/F10 melanoma combined with chronic neurogenic pain.
Methods. The study included 64 female mice. Melanoma was transplanted under the skin of the back to animals of the main group 2 weeks after the bilateral sciatic nerve ligation. Levels of NOS-3, NOS-2, endothelin-1, L-arginine, citrulline, total nitrite and ADMA were determined by ELISA in the intact skin and in tumor tissues.
Results. The study showed that the dynamics of the studied parameters differed in tumor growth alone and in combination with chronic pain. Stably increased levels of NO-synthases in the tumor and stably increased ADMA levels with their decrease by week 3 of the growth were registered in the tumor growth with pain.
Conclusions. Chronic pain probably contributes to the development of immunological tolerance to tumor antigens in the skin. Conditions are formed that facilitate the survival of tumor cells and contribute to the further development of melanoma. The dynamics of the NO-system activity can stimulate neoangiogenesis and enhance tumor invasion. Changes in the ADMA inhibitor levels in the tumor growth combined with chronic pain may indicate the control of NO levels through the metabolic regulation of NO-synthase activity, thus providing increased melanoma invasiveness.
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