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Abstract
Material and methods. The study included 65 patients with papillary thyroid cancer T1-4N0-1M0. The comparison group was represented by 39 patients with benign thyroid pathology (22 patients with follicular adenoma and 17 patients with nodular goiter). The expression of the LC3B protein was assessed using the real-time PCR method, and the protein content was assessed by the Western blotting method. The BRAF-V600E mutation was determined using the Real-time-PCR-BRAF-V600E reagent kit.
Results of the investigation. An increase in the expression and content of the LC3B protein was found in the papillary thyroid cancer in 2.7 and 3.15 times compared with patients with benign tumor pathology. Patients with tumor size T3-4N0-1M0 showed a 1.6-fold increase in the LC3B protein content compared with patients with T1-2N0M0. The defeat of regional lymph nodes in patients with PCa was associated with an increase in the expression and content of LC3B by 2.0 and 2.4 times, respectively, compared with patients without metastases. An increase in protein expression and content was revealed by 2.5 and 1.7 times, respectively, in patients with the presence of the thyroid capsule invasion compared with patients without invasion. At the same time, a high content of autophagy protein was shown in the classical variant of the tumor, where the indicator was 2.8 times higher than in the follicular subtype.
Conclusion. An increase in the expression and content of the LC3B protein in the papillary thyroid cancers was revealed. The activation of autophagy plays a decisive role in oncogenesis and in the invasive and metastatic potential formation. The autophagy activation was revealed in the classic variant of papillary thyroid cancer. The association of the BRAF-V600E mutation with the expression and content of the LC3B protein has not been shown.
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