摘要
Злокачественная опухоль представляет собой многокомпонентную пространственно сложно организованную систему, которая характеризуется индивидуальными особенностями для каждого пациента. Создание клеточной модели in vitro, максимально приближенной по структуре и свойствам к естественной опухолевой системе, позволит приблизиться к решению таких практических и теоретических задач современной онкологии, как выявление биологических закономерностей опухолевого роста, особенностей поведения клеток иммунной системы, тестирование потенциальных противоопухолевых препаратов, определение эффективности методов химио-, радио-, фотодинамической, таргетной и иммунотерапии. Такой моделью могут служить трёхмерные клеточные структуры - сфероиды (тумороиды). В обзоре представлены данные об особенностях трехмерного клеточного моделирования, характеристиках существующих 3D моделей и их пригодности в экспериментальных и клинических исследованиях.
参考
Alfarouk K.O., Verduzco D., Rauch C. et al. Glycolysis, tumor metabolism, cancer growth and dissemination. A new pH-based etiopathogenic perspective and therapeutic approach to an old cancer question // Oncoscience. - 2014. - Vol. 1. - №.12. - P 777-802.
Allen T.M. Ligand-targeted therapeutics in anticancer therapy // Nature Reviews Cancer. - 2002. - Vol. 2. - № 10. - P. 750-763.
Amann A., Zwierzina M., Gamerith G. et al. Development of an innovative 3D cell culture system to study tumour - stroma interactions in non-small cell lung cancer cells // PLoS One. - 2014. - Vol. 9. - № 3. - P e92511.
Baek N., Seo O.W., Kim M. et al. Monitoring the effects of doxorubicin on 3D-spheroid tumor cells in real-time // OncoTargets and Therapy. - 2016. - Vol. 9. - P. 7207-7218.
Baek N., Seo O.W., Lee J. Real-time monitoring of cisplatin cytotoxicity on three-dimensional spheroid tumor cells // Drug Design, Development and Therapy. - 2016. - Vol. 10. - P 2155-2165.
Bai C., Yang M., Fan Z. et al. Associations of chemo- and radio-resistant phenotypes with the gap junction, adhesion and extracellular matrix in a three-dimensional culture model of soft sarcoma // Journal of Experimental & Clinical Cancer Research. - 2015. - Vol. 34. - № 1. - P. 1-10.
Bartling B., Hofmann H.-S., Silber R.-E., Simm A. Differential impact of fibroblasts on the efficient cell death of lung cancer cells induced by paclitaxel and cisplatin // Cancer Biology & Therapy. - 2008. - Vol. 7. - № 8. - P. 1250-1261.
Blanco E., Shen H., Ferrari M. Principles of nanoparticle design for overcoming biological barriers to drug delivery // Nature Biotechnology. - 2015. - Vol. 33. - № 9. - P. 941-951.
Breslin S., O'Driscoll L. Three-dimensional cell culture: the missing link in drug discovery // Drug Discovery Today. - 2013. - Vol. 18. - № 5-6. - P. 240-249.
Bryce N.S., Zhang J.Z., Whan R.M. et al. Accumulation of an anthraquinone and its platinum complexes in cancer cell spheroids: the effect of charge on drug distribution in solid tumour models // Chemical Communications. - 2009. - Vol. 19. - P. 2673-2675.
Byrne H.M. Dissecting cancer through mathematics: from the cell to the animal model // Nature Reviews Cancer. - 2010. - Vol. 10. - № 3. - P. 221-230.
Chan H.F., Zhang Y, Ho Y-P. et al. Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment // Scientific Reports. - 2013. - Vol. 3. - № 1. - P. 3462.
Chignola R., Schenetta A., Chiesa E. et al. Oscilating growth patterns of multicellular tumor spheroids // Cell Proliferation. - 1999. - № 32. - P. 39-48.
Christensen T., Moan J., Sandquist T, Smedshammer L. Multicellular spheroids as an in vitro model system for photoradiation therapy in the presence of Hpd // Progress in clinical and biological research. - 1984. - Vol. 170. - P. 381-390.
Costa E.C., de Melo-Diogo D., Moreira A.F. et al. Spheroids Formation on Non-Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches // Biotechnology Journal. - 2018. - Vol. 13. - № 1. - P. 1-25.
Costa E.C., Gaspar V.M., Coutinho P., Correia I.J. Optimization of liquid overlay technique to formulate heterogenic 3D co-cultures models // Biotechnology and Bioengineering - 2014. - Vol. 111. - № 10. - P. 1672-1685.
Courau T., Bonnereau J., Chicoteau J. et al. Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment // Journal for ImmunoTherapy of Cancer. - 2019. - Vol. 7. - № 1. - P. 1-14.
de Ridder L., Cornelissen M., de Ridder D. Autologous spheroid culture: a screening tool for human brain tumour invasion // Critical Reviews in Oncology/Hematology. - 2000. - Vol. 36. - № 2. - P. 107-122.
Deakin A.S. Model for the Growth of a solid in vitro tumor // Growth. - 1975. - Vol. 39. - P. 159-165.
Doix B., Bastien E., Rambaud A. et al. Preclinical Evaluation of White Led-Activated Non-porphyrinic Photosensitizer OR141 in 3D Tumor Spheroids and Mouse Skin Lesions // Frontiers in Oncology. - 2018. - Vol. 8. - P. 1-9.
Friedrich J., Ebner R., Kunz-Schughart L.A. Experimental anti-tumor therapy in 3-D: spheroids - old hat or new challenge? // International Journal of Radiation Biology. - 2007. - Vol. 83. - № 11-12. - P. 849-871.
Friedrich J., Seidel C., Ebner R., Kunz-Schughart L.A. Spheroid-based drug screen: considerations and practical approach // Nature Protocols. - 2009. - Vol. 4. - № 3. - P. 309-324.
Furbert-Harris P.M., Laniyan I., Harris D. et al. Activated eosinophils infiltrate MCF-7 breast multicellular tumor spheroids // Anticancer research. - 2003. - Vol. 23. - № 1A. - P. 71-78.
Goodman T.T., Olive P.L., Pun S.H. Increased nanoparticle penetration in collagenase-treated multicellular spheroids // International Journal of Nanomedicine. - 2007. - Vol. 2. - P. 265-274.
Guller A.E., Grebenyuk P.N., Shekhter A.B. et al. Bioreactor-Based Tumor Tissue Engineering // Acta Naturae. - 2016. - Vol. 8. - № 3. - P. 44-58.
Gunther S., Ruhe C., Derikito M. G. et al. Polyphenols prevent cell shedding from mouse mammary cancer spheroids and inhibit cancer cell invasion in confrontation cultures derived from embryonic stem cells // Cancer Letters. - 2007. - Vol. 250. - № 1. - P. 25-35.
Haq S., Samuel V., Haxho F. et al. Sialylation facilitates self-assembly of 3D multicellular prostaspheres by using cyclo-RGDfK(TPP) peptide // OncoTargets and Therapy. - 2017. - Vol. 10 - P. 2427-2427.
Harris A.L. Hypoxia - a key regulatory factor in tumour growth // Nature Reviews Cancer. - 2002. - Vol. 2. - № 1. - P. 38-47.
Heimdal J.H., Aarstad H.J., Olsnes C., Olofsson J. Human autologous monocytes and monocyte-derived macrophages in co-culture with carcinoma F-spheroids secrete IL-6 by a non-CD14-dependent pathway // Scandinavian Journal of Immunology. - 2001. - Vol. 53. - № 2. - P. 162-170.
Hirschhaeuser F., Menne H., Dittfeld C. et al. Multicellular tumor spheroids: an underestimated tool is catching up again // Journal of Biotechnology. - 2010. - Vol. 148. - № 1. - P. 3-15.
Holtfreter J. A study of the mechanics of gastrulation // J. Exp. Zool. - 1944. - Vol. 95. - P. 171-212.
Ivanov D. P., Parker T. L., Walker D. A. et al. Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres // PLoS ONE. - 2014. - Vol. 9. - № 8. - P. e103817.
Ivascu A., Kubbies M. Diversity of cell-mediated adhesions in breast cancer spheroids // International Journal of Oncology. - 2007. - Vol. 31. - P. 1403-1413.
Iyer N.V., Kotch L.E., Agani F. et al. Cellular and developmental control of O2 of hypoxia-inducible factor 1 // Genes & Development. - 1998. - Vol. 12. - № 2. - P. 149-162.
Kim J., Dang, C.V. Cancer's Molecular Sweet Tooth and the Warburg Effect // Cancer Research. - 2006. - Vol. 66. - № 18. - P. 8927-8930.
Knuchel R., Hofstadter F., Jenkins W.E., Masters J.R. Sensitivities of monolayers and spheroids of the human bladder cancer cell line MGH-U1 to the drugs used for intravesical chemotherapy // Cancer Research. - 1989. - Vol. 49. - P. 1397-1401.
Konur A., Kreutz M., Knuchel R. et al. Three-dimensional co-culture of human monocytes and macrophages with tumor cells: analysis of macrophage differentiation and activation // International Journal of Cancer. - 1996. - Vol. 66. - № 5. - P. 645-652.
Kostarelos K., Emfietzoglou D., Papakostas A. Sgouros, Engineering lipid vesicles of enhanced intratumoral transport capabilities: correlating liposome characteristics with penetration into human prostate tumor spheroids // Journal of Liposome Research. - 2005. - Vol. 15. - № 1. - P. 15-27.
Kozin S.V., Gerweck L.E. Cytotoxicity of weak electrolytes after the adaptation of cells to low pH: role of the transmembrane pH gradient // British Journal of Cancer - 1998. - Vol. 77. - № 10. - P. 1580-1585.
Kuh H.J., Jang S.H., Wientjes M. G. et al. Determinants of paclitaxel penetration and accumulation in human solid tumor // Journal of Pharmacology and Experimental Therapeutic. -1999. - Vol. 290. - № 2. - P. 871-880.
Kunz-Schughart L.A., Freyer J.P., Hofstaedter F., Ebner R. The use of 3-D cultures for high-throughput screening: the multicellular spheroid model // Journal of Biomolecular Screening. - 2004. - Vol. 9. - № 4. - P. 273-285.
Kunz-Schughart L.A., Kreutz M., Knuechel R. Multicellular spheroids: a three-dimensional in vitro culture system to study tumour biology // International Journal of Experimental Pathology. - 1998. - Vol. 79. - № 1. - P. 1-23.
L'Esperance S., Bachvarova M., Tetu B. et al. Global gene expression analysis of early response to chemotherapy treatment in ovarian cancer spheroids // BMC Genomics. - 2008. - Vol. 9. - № 1. - P. 99.
Lee H., Osanai M., Tokusashi V. Morphology, proliferation and apoptosis of mouse liver epithelial cells cultured as spheroids // Japanese Journal of Cancer Research. - 1999. - Vol. 90. - № 10. - P.1109-1116.
Lin R.Z., Chou L.-F., Chien C.-C.M., Chang H.-Y Dynamic analysis of hepatoma spheroid formation: roles of E-cadherin and 1-integrin // Cell and Tissue Research. - 2006. - Vol. 324. - № 3. - P. 411-422.
Lin R.Z., Chang H.Y Recent advances in three-dimensional multicellular spheroid culture for biomedical research // Biotechnology Journal. - 2008. - Vol. 3. - № 9-10. - P. 1172-1184.
Lu S., Zhao F., Zhang Q., Chen, P. Therapeutic Peptide Amphiphile as a Drug Carrier with ATP-Triggered Release for Synergistic Effect, Improved Therapeutic Index, and Penetration of 3D Cancer Cell Spheroids // International Journal of Molecular Sciences. - 2018. - Vol. 19. - № 9. - P. 1-14.
Ludwig K., Tse E.S., Wang J.Y Colon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors // BMC Cancer. - 2013. - Vol. 13. - P. 221.
Madsen S.J., Sun C.-H., Tromberg B.J. et al. Effects of combined photodynamic therapy and ionizing radiation on human glioma spheroids // Photochemistry and Photobiology. - 2002. - Vol. 76. - № 4. - P. 411-416.
Majety M., Pradel L.P., Gies M., Ries C.H. Fibroblasts influence survival and therapeutic response in a 3D coculture model // PLoS One. - 2015. - Vol. 10. - P. e0127948.
Martin A. R., Ronco C., Demange L., Benhida R. Hypoxia inducible factor down-regulation, cancer and cancer stem cells (CSCs): ongoing success stories // MedChem-Comm. - 2017. - Vol. 8. - № 1. - P. 21-52.
Mehta G., Hsiao A.Y, Ingram M., Luker G.D., Takayama S. Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy // Journal of Controlled Release. - 2012. - Vol. 164. - № 2. - P.192-204.
Minchinton, A.I., Tannock, I.F. Drug penetration in solid tumours // Nature Reviews Cancer. - 2006. - Vol. 6. - № 8. - P. 583-592.
Miranti C.K., Brugge J.S. Sensing the environment: a historical perspective on integrin signal transduction // Nature Cell Biology. - 2002. - Vol. 4. - № 4. - P. 83-90.
Nichikawa V., Torkusashi V., Kodahama T. et al. Hepatocytic cells form bile duct-like structures within a three dimensional collagen gel matrix // Experimental Cell Research. - 1996. - № 223. - P. 357-371.
Oudar O. Spheroids: relation between tumour and endothelial cells // Critical Reviews in Oncology/Hematology. - 2000. - Vol. 36. - № 2. - P. 99-106.
Pauli C., Hopkins B.D., Prandi D. et al. Personalized in vitro and in vivo cancer models to guide precision medicine // Cancer Discovery. - 2017. - Vol. 7. - № 5. - P. 462-477.
Quail D.F., Joyce J.A. Microenvironmental regulation of tumor progression and metastasis // Nature Medicine. - 2013. - Vol. 19. - № 11. - P. 1423-1437.
Raica M., Cimpean A.M., Ribatti D. Angiogenesis in pre-malignant conditions // European Journal of Cancer. - 2009. - Vol. 45. - № 11. - P. 1924-1934.
Sachs N., Clevers H. Organoid cultures for the analysis of cancer phenotypes // Current Opinion in Genetics & Development - 2014. - Vol. 24. - P. 68-73.
Schwartz D.K., Dotson R.S. Dynamics of spheroid selfassembly in liquid-overlay culture of DU 145 human prostate cancer cells // Biotechnology and Bioengineering. -2001. - Vol.72. - № 6. - P. 579-591.
Shankar S., Nall D., Tang S.-N. et al. Resveratrol inhibits pancreatic cancer stem cell characteristics in human and KrasG12D transgenic mice by inhibiting pluripotency maintaining factors and epithelial-mesenchymal transition // PLoS One. - 2011. - Vol. 6. - P. e16530.
Shi C., Cao H., He W. et al. Novel drug delivery liposomes targeted with a fully human anti-VEGF165 monoclonal antibody show superior antitumor efficacy in vivo // Biomedicine & Pharmacotherapy. - 2015. - Vol. 73. - P. 48-57.
Smyrek I., Mathew B., Fischer S.C. et al. E-cadherin, actin, microtubules and FAK dominate different spheroid formation phases and important elements of tissue integrity // Biol Open. - 2019. - Vol. 8. - № 1.
St Croix B., Man S., Kerbel R.S. Reversal of intrinsic and acquired forms of drug resistance by hyaluronidase treatment of solid tumors // Cancer Letters. - 1998. - Vol. 131. - № 1. - P. 35-44.
Sutherland R.M., Durand R.E., Jones W.B.G. Modification by triacetoneamine-N-oxyl (TAN) of the radiation response of chinese hamster cells grown as a tumor model // Radiation Research. - 1971. - Vol. 47. - P. 342.
Sutherland R.M., Inch W.R., McCredie J.A., Kruuv J. A multicomponent radiation survival curve using an in vitro tumour model // International journal of radiation biology and related studies in physics, chemistry, and medicine. - 1970. - Vol. 18. - № 5. - P. 491-495.
Tannock I.F., Lee C.M., Tunggal J.K. et al. Limited penetration of anticancer drugs through tumor tissue: a potential cause of resistance of solid tumors to chemotherapy // Clinical Cancer Research. - 2002. - Vol. 8. - P. 878-884.
Tannock I.F., Rotin D. Acid pH in tumors and its potential for therapeutic exploitation // Cancer Research. -1989. - Vol. 49. - № 16. - P. 4373-4384.
Tredan O., Galmarini C.M., Patel K., Tannock I.F. Drug resistance and the solid tumor microenvironment // JNCI Journal of the National Cancer Institute. - 2007. - Vol. 99. - P. 1441-1454.
Tzanakakis E.S., Hansen L.K., Hu W.-S. The role of actin filaments and microtubules in hepatocyte spheroid self-assembly // Cell Motility and the Cytoskeleton. - 2001. - Vol. 48. - P. 175-189.
van Dijk M., Goransson S.A., Stromblad S. Cell to extracellular matrix interactions and their reciprocal nature in cancer // Experimental Cell Research. - 2013. - Vol. 319. - № 11. - P. 1663-1670.
Vaupel P. The role of hypoxia-induced factors in tumor progression // The Oncologist. - 2004. - Vol. 9. - P. 10-17.
Vogel S., Peters C., Etminan N. et al. Migration of mesenchymal stem cells towards glioblastoma cells depends on hepatocyte-growth factor and is enhanced by amino-laevulinic acid-mediated photodynamic treatment // Biochemical and Biophysical Research Communications. - 2013. - Vol. 431. - № 3. - P. 428-432.
Vugas J.M., Tarleton A.E., Moizen K.B., Molzen K.B. Multicellular tumor spheroids formation by breast cancer cells isolated from different sites // Cancer Research. - 1978. - Vol. 38. - P. 2486-2491.
Ward J.P., King J. Mathematical modelling of avascular-tumour growth // Mathematical Medicine and Biology. - 1997. - Vol. 14. - № 1. - P. 39-69.
Wartenberg M., Gronczynska S., Bekhite M.M. et al. Regulation of the multidrug resistance transporter P-glycoprotein in multicellular prostate tumor spheroids by hyperthermia and reactive oxygen species // International Journal of Cancer. - 2005. - Vol. 113. - P. 229-240.
Wartenberg M., Hoffmann E., Schwindt H. Reactive oxygen species-linked regulation of the multidrug resistance transporter P-glycoprotein in Nox-1 overexpressing prostate tumor spheroids // FEBS Letters. - 2005. - Vol. 579. - № 20. - P. 4541-4549.
Weng K.C., Hashizume R., Noble C.O. et al. Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models // Nanomedicine. - 2013. - Vol. 8. - № 12. - № 1913-1925.
West C.M., Moore J.V. Flow cytometric analysis of intracellular hematoporphyrin derivative in human tumour cells and multicellular spheroids // Photochemistry and Photobiology. - 1989. - Vol. 50. - № 5. - P. 665-669.
West C.M., Moore J.V. Mechanisms behind the resistance of spheroids to photodynamic treatment: A flow cytometric study // Photochemistry and Photobiology. - 1992. - Vol. 55. - № 3. - P. 425-430.
Ying X., Wen H., Lu W.L et al. Dual-targeting daunorubicin liposomes improve the therapeutic efficacy of brain glioma in animals // Journal of Controlled Release. - 2010. - Vol. 141. - № 2. - P. 183-192.
Yokoi K., Tanei T., Godin B. et al. Serum biomarkers for personalization of nanotherapeutics-based therapy in different tumor and organ microenvironments // Cancer Letters. - 2014. - Vol. 345. - № 1. - P. 48-55.
Yoshii Y, Waki A., Yoshida K. et al. The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation // Biomaterials - 2011. - Vol. 32. - P. 6052-6058.
Zhu C., Sempkowski M., Holleran T. et al. Alpha-particle radiotherapy: For large solid tumors diffusion trumps targeting // Biomaterials. - 2017. Vol. 130. - P. 67-75.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
© АННМО «Вопросы онкологии», Copyright (c) 2019