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Abstract
Introduction. Endometrial cancer is the leading oncogynecological pathology in Russia. Proteasomes, multisubunit proteolytic complexes, play an important role in the development of endometrial cancer.
Aim. Evaluation of the prognostic significance of proteasome activity and the level of immune subunits of proteasomes LMP2 and LMP7 in relation to overall and disease-free survival in endometrial cancer.
Materials and methods. The study included 90 patients with endometrial cancer, stage I-III. Chymotrypsin-like activity of proteasomes was determined in clarified homogenates of tumor and intact endometrial tissue by hydrolysis of the fluorogenic oligopeptide Suc-LLVY-AMC (Sigma). The content of LMP2 and LMP7 immune subunits of the proteasomes was determined by Western blotting. Results. Аn increase in the chymotrypsin-like activity of proteasomes in the tumor tissue was revealed compared to the unchanged endometrium, as well as in stage III tumors compared to stages I and II. In the endometrial cancer tissue, an increase in the content of LMP2 and LMP7 subunits was observed in relation to the unchanged tissue. One-way analysis of proteasome parameters showed that chymotrypsin-like activity of proteasomes is associated with 3-year relapse-free survival of patients with endometrial cancer, and the content of the proteasome LMP2 subunit is associated with overall survival.
Conclusion. The chymotrypsin-like activity of proteasomes and the content of the LMP2 subunits in the tumor tissue can be considered as additional criteria for recurrence and overall survival, which in turn will allow planning an adequate amount of treatment for patients.
References
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