Vol. 70 No. 2 (2024), EDITORIALS

Vol. 70 No. 2 (2024)

Possibilities for Differential Diagnosis of Thyroid Follicular Neoplasia by Evaluation of Small Noncoding RNA

EDITORIALS

https://doi.org/10.37469/0507-3758-2024-70-2-189-201

Published 06.05.2024

Ioannina A. Bandyk

Algimed-Techno LLC, Minsk, the Republic of Belarus

https://orcid.org/0000-0001-6148-6778

Margarita S. Knyazeva

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0002-2079-5061

Alexander Yu. Garanin

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0002-8313-3794

Konstantin E. Katsuba

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0002-7373-5206

Lidia M. Zabegina

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0003-0827-1641

Tatyana V. Sharonova

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0002-5372-1253

Andrey V. Shalaev

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0002-6148-6994

Ekaterina Ya. Grinkova

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0009-0002-7238-5870

Daniil S. Plevako

N.N. Petrov National Medicine Research Center of Oncology, St. Petersburg, the Russian Federation

https://orcid.org/0000-0002-5657-130X

Анна Олеговна Нюганен

ФГБУ «НМИЦ онкологии им. Н.Н Петрова»

https://orcid.org/0000-0003-2685-5093

Ирина Андреевна Полковникова

СПб ГБУЗ «Городская больница № 40»

https://orcid.org/0000-0002-9178-8512

Анна Сергеевна Артемьева

ФГБУ «НМИЦ онкологии им. Н.Н Петрова»

https://orcid.org/0000-0002-2948-397X

Светлана Вячеславовна Апалько

СПб ГБУЗ «Городская больница № 40»

https://orcid.org/0000-0002-3853-4185

Сергей Григорьевич Щербак

СПб ГБУЗ «Городская больница № 40»

https://orcid.org/0000-0001-5047-2792

Анастасия Валерьевна Малек

ФГБУ «НМИЦ онкологии им. Н.Н Петрова»

https://orcid.org/0000-0001-5334-7292

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##article.numberofviews## 188

pdf (Русский)

Keywords

ollicular thyroid cancer
preoperative diagnosis
next generation sequencing
microRNA
piwiRNA

How to Cite

Bandyk , I. A., Knyazeva , M. S., Garanin , A. Y., Katsuba , K. E., Zabegina, L. M., Sharonova , T. V., Shalaev , A. V., Grinkova , E. Y., Plevako , D. S., Нюганен, А. О., Полковникова, И. А., Артемьева, А. С., Апалько, С. В., Щербак, С. Г., & Малек, А. В. (2024). Possibilities for Differential Diagnosis of Thyroid Follicular Neoplasia by Evaluation of Small Noncoding RNA. Voprosy Onkologii, 70(2), 189–201. https://doi.org/10.37469/0507-3758-2024-70-2-189-201

Abstract

Introduction. The preoperative differential diagnosis of follicular thyroid nodules is not a trivial issue due to the absence of objective signs of malignancy. This is stimulating efforts to find molecular markers of follicular cancer and to develop diagnostic test systems. Small regulatory RNAs are a large group of molecules that perform different biological functions, but have a similar biochemical structure. Quantitative analysis of different members of this group can be performed simultaneously using identical technology. This determines the possibility of a comprehensive assessment of the cellular biology of the samples analysed and the development of new diagnostic criteria. The purpose of the study was a comparative analysis of small RNAs expression profile in the samples of follicular thyroid cancer and follicular adenoma of the thyroid gland.

Materials and methods. The study included tissue samples of follicular carcinoma (FC, n = 12) and benign follicular adenoma (FA, n = 12) of the thyroid gland obtained after thyroidectomy and histological examination. Analysis of the expression profile of short RNAs was carried out using next generation sequencing.

Results. The expression (concentration) of transfer RNAs (tRNAs), small nuclear RNAs (snRNAs), and a large group of unclassified molecules (miscRNA) is increased in FC compared to FA, but the diagnostic potential of individual molecules is relatively low. The total concentrations of microRNA molecules (miRNA) turned out to be comparable in the FC and FA groups; analysis of «reciprocal pairs» of miRNA markers allowed to differentiate FC and FA with a high degree of probability (AUC: 0.94-0.98). The total number of piwiRNA molecules was slightly higher in FC samples compared to FA; analysis of «reciprocal pairs» of piwiRNA marker allows us to reliably differentiate FC and FA (AUC: 1.00).

Conclusion. Analysis of the concentration of marker miRNA and piwiRNA in fine-needle aspiration biopsy material appears to be a promising method for additional diagnosis of thyroid nodules with a follicular structure.

https://doi.org/10.37469/0507-3758-2024-70-2-189-201

##article.numberofdownloads## 113

##article.numberofviews## 188

pdf (Русский)

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